| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.204686 |
| Chromosome: | chromosome 2 |
| Location: | 1649600 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085450 | CPX1 | (1 of 2) 1.3.3.3 - Coproporphyrinogen oxidase / Coproporphyrinogenase; Coproporphyrinogen III oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGATGGGCATGCGGGTGGGCGAGGCGGCA |
| Internal bar code: | TTTTCACAGTTGACCTTTGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 807 |
| LEAP-Seq percent confirming: | 97.1881 |
| LEAP-Seq n confirming: | 7984 |
| LEAP-Seq n nonconfirming: | 231 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGCAGTCTGCTTTGTGCT |
| Suggested primer 2: | CCGCTACTTCGAGACTGAGG |