Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.204747 |
Chromosome: | chromosome_12 |
Location: | 4009401 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g516950 | SAS6 | Basal body cartwheel protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTCCAGCAGGTGCTTGTCGTACTGCTCACG |
Internal bar code: | ACGTATTAATGGGCATAGCGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 505 |
LEAP-Seq percent confirming: | 99.6259 |
LEAP-Seq n confirming: | 1864 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAACCCTAGAAGCCAACAA |
Suggested primer 2: | TCCGTGTTCAAGATTGTGGA |