Insertion junction: LMJ.RY0402.204760_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.204760
Chromosome:chromosome 2
Location:428784
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g076150 (1 of 1) PTHR13617//PTHR13617:SF14 - FAMILY NOT NAMED // PROTEIN C54G4.7 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCGCGCCACACGCCTACGTGTGCCGCTC
Internal bar code:GCCTATGGGGGGACGCAGTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:841
LEAP-Seq percent confirming:99.7286
LEAP-Seq n confirming:6247
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:AAAATGTACCCAACCCCACA
Suggested primer 2:AACAGGACCACATGCACAGA