Insertion junction: LMJ.RY0402.204762_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.204762
Chromosome:chromosome 11
Location:3260301
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g479850 CYN59 Cyclophilin 59; (1 of 1) K12735 - peptidyl-prolyl cis-trans isomerase-like 4 [EC:5.2.1.8] (PPIL4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTTACCGCCCCGTACCCACCCCATCCCTC
Internal bar code:CGGTGGCGCGGGGTATTTGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:416
LEAP-Seq percent confirming:74.2574
LEAP-Seq n confirming:75
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GTCACGTTCACGCTCACG
Suggested primer 2:ACTGGACCACCACCTAGCAC