| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.204787 |
| Chromosome: | chromosome 7 |
| Location: | 3792706 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338400 | (1 of 1) K14845 - RAT1-interacting protein (RAI1, DOM3Z) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGGCTCCGAGTTGGACTTGACGTGGAC |
| Internal bar code: | CTTTTCCCAACGCACGGCCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 667 |
| LEAP-Seq percent confirming: | 99.4889 |
| LEAP-Seq n confirming: | 584 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCTCTTCCTCCAGCCTGT |
| Suggested primer 2: | CATCAGTACGACATCCCGTG |