Insertion junction: LMJ.RY0402.204805_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.204805
Chromosome:chromosome 3
Location:2882471
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g162750 (1 of 2) K13207 - CUG-BP- and ETR3-like factor (CUGBP, BRUNOL, CELF) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGCTGTGTGTCGGGGTCTGCATCGTACA
Internal bar code:AGCCTGTCGACACAGGTGCTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:670
LEAP-Seq percent confirming:93.0512
LEAP-Seq n confirming:8356
LEAP-Seq n nonconfirming:624
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCTATACGTCGATTTGGT
Suggested primer 2:GAAACGACACGAGATGACGA