Insertion junction: LMJ.RY0402.204814_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.204814
Chromosome:chromosome 12
Location:8421818
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g548550 (1 of 1) PF05742 - Transport and Golgi organisation 2 (TANGO2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCAGCAGGCGGCGGCGCGGGCAGCTGCAC
Internal bar code:ATCATAGCTGGTCCGTGTGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:686
LEAP-Seq percent confirming:26.087
LEAP-Seq n confirming:66
LEAP-Seq n nonconfirming:187
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GATGAAACATGCCGAAACAG
Suggested primer 2:TTGTCACAGTCTCTGGACGC