| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.204862 |
| Chromosome: | chromosome_1 |
| Location: | 4311750 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g029150 | DAU1,ODA7 | Outer row dynein assembly protein, predominantly cytoplasmic | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCAAGGGGTTCTCACAGATTGCCTGTCTT |
| Internal bar code: | GAAGGCCGGTTGGAGCCTACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1014 |
| LEAP-Seq percent confirming: | 99.7215 |
| LEAP-Seq n confirming: | 358 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAAGCCAGGTCATCAAGC |
| Suggested primer 2: | GTTACAACAGGTCCGCAGGT |