| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.204863 |
| Chromosome: | chromosome 3 |
| Location: | 1406899 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g151000 | SEX4,DSP8 | Laforin glucan phosphatase, chloroplastic; (1 of 1) PTHR10343//PTHR10343:SF58 - 5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT // PHOSPHOGLUCAN PHOSPHATASE DSP4, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAAGAGAACACGCGGGACTGTGCCTGCG |
| Internal bar code: | ACCGTGCCACACGCAACACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 530 |
| LEAP-Seq percent confirming: | 99.5875 |
| LEAP-Seq n confirming: | 2414 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCTGTCGTCGAAGTCAAT |
| Suggested primer 2: | CAGACTTCAAGGTGGGGAGA |