| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.204865 |
| Chromosome: | chromosome 9 |
| Location: | 5398596 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399960 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGGAGGGCCGAGCGGCAGCAGCAGCCCT |
| Internal bar code: | CCTGCCAATCCCTGCTTTCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 99.4885 |
| LEAP-Seq n confirming: | 389 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGGAGGTCCGCCTTGTAT |
| Suggested primer 2: | GCACTGTGCCAAGTTTGAGA |