| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.204881 |
| Chromosome: | chromosome 12 |
| Location: | 1884598 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g511350 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAAGCATCGGCTATGGGGCGGGCGAAGTT |
| Internal bar code: | GCGCGTATGAGTTTAGCGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 995 |
| LEAP-Seq percent confirming: | 99.3927 |
| LEAP-Seq n confirming: | 3928 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCTAGGTATCATGGCGG |
| Suggested primer 2: | TTGAAGCTTGCTGGGAGAAT |