| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.204884 |
| Chromosome: | chromosome 12 |
| Location: | 8997634 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g543850 | (1 of 1) PF09497 - Transcription mediator complex subunit Med12 (Med12) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCGCTGCACTGTGTGCGCCCGGCCCGC |
| Internal bar code: | AAGCGTAGATTACCGCGCGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 770 |
| LEAP-Seq percent confirming: | 99.571 |
| LEAP-Seq n confirming: | 4642 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACAAGCGCAGCAGCAGTA |
| Suggested primer 2: | AGGTCCGTACGGGCTCTTAT |