| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.204888 |
| Chromosome: | chromosome 12 |
| Location: | 3910460 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515950 | (1 of 35) IPR001763 - Rhodanese-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGGAAGAGGCCGCCAGAATCAAGGCGG |
| Internal bar code: | TCTATTTTTGAATGTCCGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 209 |
| LEAP-Seq percent confirming: | 99.4462 |
| LEAP-Seq n confirming: | 5028 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGCTTGCGCCTTAGC |
| Suggested primer 2: | CGGTTAAGATAGGCGCAGAG |