| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.204889 |
| Chromosome: | chromosome 7 |
| Location: | 302907 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314300 | (1 of 1) PF07092 - Protein of unknown function (DUF1356) (DUF1356) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGGCAGTGTGGCATGTTACTGCCGTGAG |
| Internal bar code: | TCTGATTACTCTGGGGGACCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 477 |
| LEAP-Seq percent confirming: | 99.261 |
| LEAP-Seq n confirming: | 4567 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGGATTGGGAGGAGCTT |
| Suggested primer 2: | CCCGTGTGTGTGTCTAGTGG |