| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.204897 |
| Chromosome: | chromosome 16 |
| Location: | 5551930 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680200 | (1 of 1) PTHR31419//PTHR31419:SF1 - FAMILY NOT NAMED // AUXIN EFFLUX CARRIER FAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAAGGCCTTGGTATCAGTGTGAGGATGT |
| Internal bar code: | CCCGAACATGGCGAATTGTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 914 |
| LEAP-Seq percent confirming: | 99.6905 |
| LEAP-Seq n confirming: | 6442 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGATGTCCACCATCCTG |
| Suggested primer 2: | GCTCTCACGCACCCTTACTC |