Insertion junction: LMJ.RY0402.204914_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.204914
Chromosome:chromosome 6
Location:4309215
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278257 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACTCCGTCCCTTGTGCTCTCAATAG
Internal bar code:ATTGAAGCGGTTAAAATCTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:867
LEAP-Seq percent confirming:99.8162
LEAP-Seq n confirming:3258
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTGACGACAGCCCTCACT
Suggested primer 2:CTGTAAGAGGCTGGTCCGAG