| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.204926 |
| Chromosome: | chromosome 2 |
| Location: | 2288462 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g090500 | HEL10 | (1 of 1) K13131 - ATP-dependent RNA helicase DDX20 (DDX20, GEMIN3); DEAD box ATP-dependent RNA helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATTAGTCAAGGAAGTCCATGATAAAGA |
| Internal bar code: | GGTTCTGGGTGGGGTCGGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 319 |
| LEAP-Seq percent confirming: | 86.8182 |
| LEAP-Seq n confirming: | 191 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTCCCCAGAATGTTGAGT |
| Suggested primer 2: | CCCGTACAAGTCCTCTGGAA |