| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.204947 |
| Chromosome: | chromosome_13 |
| Location: | 1493701 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g572650 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAATACTACCCGAAACGAGGCACGAGCCCT |
| Internal bar code: | TTAAAAAACGTATGAAAACTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 368 |
| LEAP-Seq percent confirming: | 96.4286 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGAGGACATGTCAAAACG |
| Suggested primer 2: | GAGCTGGTACTGCTGGAAGG |