| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.204964 |
| Chromosome: | chromosome 4 |
| Location: | 1252892 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215100 | SSS1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCATGTTCGCACAGCGAACCGGCGTGAT |
| Internal bar code: | CCATGTCGTCGCCGTTTCGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 893 |
| LEAP-Seq percent confirming: | 99.5712 |
| LEAP-Seq n confirming: | 2554 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACAGGACACACGACACC |
| Suggested primer 2: | TAAGCGTTTCACTGGACGTG |