| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.204967 |
| Chromosome: | chromosome 6 |
| Location: | 2124739 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g265602 | (1 of 1) IPR006569//IPR008942//IPR009010 - CID domain // ENTH/VHS // Aspartate decarboxylase-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCGCGGCAATATCTCTCACCTGATGAGT |
| Internal bar code: | CGCTCTGTGACCTGATCGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 880 |
| LEAP-Seq percent confirming: | 99.0066 |
| LEAP-Seq n confirming: | 1196 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGAGTATTCGGGGACAGG |
| Suggested primer 2: | GACTTTGGCAGCTTCAGTCC |