| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.204968 |
| Chromosome: | chromosome_2 |
| Location: | 7863513 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g141700 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCGTCTCCTGCGCTGTCGCCGAGCTCCTT |
| Internal bar code: | GTAGAAGTTCTAAGGGGATCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 833 |
| LEAP-Seq percent confirming: | 99.4567 |
| LEAP-Seq n confirming: | 5126 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGGTGTTGGGCTGATACT |
| Suggested primer 2: | GACACACTGGGGGCTACACT |