| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.204972 |
| Chromosome: | chromosome 17 |
| Location: | 6913028 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g745847 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCGGCCCAGAGCAGTTCCGCCAGCTTG |
| Internal bar code: | GGCCACATGCAAGACGCCGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 313 |
| LEAP-Seq percent confirming: | 98.8618 |
| LEAP-Seq n confirming: | 608 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACACATCGGCATTACGTC |
| Suggested primer 2: | CTTAGTCACCGGCAGCTTTC |