Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.204982 |
Chromosome: | chromosome_4 |
Location: | 2518387 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g222050 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCGTGGTTGGGCGTAGCTGGATGCGGCGGC |
Internal bar code: | GCGTTGGTCACCAGACGGGGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 599 |
LEAP-Seq percent confirming: | 99.7234 |
LEAP-Seq n confirming: | 5769 |
LEAP-Seq n nonconfirming: | 16 |
LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTTGGTTTAGTTTGGGCTGG |
Suggested primer 2: | CAGCCTGCACACAGGAAATA |