| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.204982 |
| Chromosome: | chromosome 4 |
| Location: | 2518387 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g222050 | (1 of 3) K16489 - protein SFI1 (SFI1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGGTTGGGCGTAGCTGGATGCGGCGGC |
| Internal bar code: | GCGTTGGTCACCAGACGGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 599 |
| LEAP-Seq percent confirming: | 99.7234 |
| LEAP-Seq n confirming: | 5769 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTTAGTTTGGGCTGG |
| Suggested primer 2: | CAGCCTGCACACAGGAAATA |