Insertion junction: LMJ.RY0402.205009_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.205009
Chromosome:chromosome 14
Location:3711834
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g631950 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATCGCGCTTTGCGGGCCATTTCGCCCAA
Internal bar code:AAGAGTTGATTATTGATTGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:861
LEAP-Seq percent confirming:99.8252
LEAP-Seq n confirming:1713
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CAAACCAGAACCCTCTTCCA
Suggested primer 2:CACAAAGCCACAGAAAGCAA