Insertion junction: LMJ.RY0402.205020_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.205020
Chromosome:chromosome 3
Location:5782317
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g188750 HEL21 (1 of 1) K13177 - ATP-dependent RNA helicase DDX1 [EC:3.6.4.13] (DDX1); DEAD/DEAH box helicase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCCGCTCTCCACGAAGTCGATCACGCGG
Internal bar code:GGACGCAAGCAAGAAACCGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:147
LEAP-Seq percent confirming:99.3548
LEAP-Seq n confirming:154
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGCGAGCCAACATCACTA
Suggested primer 2:CGCGCTGTTTCTGTTTATGA