| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.205035 |
| Chromosome: | chromosome 10 |
| Location: | 3988926 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448850 | FAP135,ODA5AK,CAL5 | (1 of 3) 3.4.22.54 - Calpain-3 / Muscle-specific calcium-activated neutral protease 3; Calpain-Like Flagellar Associated Protein 135 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAGGTGGCCTCGTCTTCCCAGCTGTCTG |
| Internal bar code: | GTCGTGAGGGCACGGGTTCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 323 |
| LEAP-Seq percent confirming: | 99.7615 |
| LEAP-Seq n confirming: | 3764 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAAGACAGACACGCCAGA |
| Suggested primer 2: | CTTCTTGACAGTCATGCGGA |