| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.205040 |
| Chromosome: | chromosome 9 |
| Location: | 1712346 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396800 | CSE19 | Predicted protein of CSE family | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGCGACAAAACTGACTGGCTCAGATTTT |
| Internal bar code: | TAGTCTAATTTTCAAGATTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 34 |
| LEAP-Seq percent confirming: | 68.75 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAGGATGCTACGAGGTC |
| Suggested primer 2: | CTGCCGGGTTTGTACTTCAT |