| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205091 |
| Chromosome: | chromosome 3 |
| Location: | 7759657 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g203351 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGTCTTCACAGGCCGCGGGGCAGGATT |
| Internal bar code: | CTAACCGCGGCTCGACGCGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 837 |
| LEAP-Seq percent confirming: | 94.4896 |
| LEAP-Seq n confirming: | 1629 |
| LEAP-Seq n nonconfirming: | 95 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGATAAGCACGAGTTGGCA |
| Suggested primer 2: | AATACCACTGCCGCCACTAC |