Insertion junction: LMJ.RY0402.205094_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.205094
Chromosome:chromosome 17
Location:1565116
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g707551 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCTACTTCGTACCCGTGGAAGGGGTTGA
Internal bar code:CCGAGACGAAGGTCACCGGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:959
LEAP-Seq percent confirming:93.1384
LEAP-Seq n confirming:828
LEAP-Seq n nonconfirming:61
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTCCCACCATCACTCTTG
Suggested primer 2:ACTACCTGCGCTTGAGGTGT