| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205095 |
| Chromosome: | chromosome 7 |
| Location: | 6083462 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355433 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTTGCAGCACAGGGTGCACTGCGCTTA |
| Internal bar code: | TACTTATGCTGGGCCGGCTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 580 |
| LEAP-Seq percent confirming: | 99.8703 |
| LEAP-Seq n confirming: | 2310 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAATTCCTTCCCTTCCTC |
| Suggested primer 2: | TGCGCGCTAAACACTTATTG |