Insertion junction: LMJ.RY0402.205110_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.205110
Chromosome:chromosome_7
Location:1012347
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g319350 sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):GTCTTGACGTGGGTCGGCGCCGCTTGCGGA
Internal bar code:CGGCAGGGACAACGCGCGGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:109
LEAP-Seq percent confirming:59.5745
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGAACTTGCTCAGTGCGGAT
Suggested primer 2:CTTCGTCTGCAGCACCATAA