| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205131 |
| Chromosome: | chromosome 16 |
| Location: | 5886879 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677300 | GOX16 | Glyoxal oxidase 16; (1 of 12) 1.1.3.9 - Galactose oxidase / Beta-galactose oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTTGGGTGGCATATGCTGCTGAGGCGC |
| Internal bar code: | GGCCATCCCGTCGTCTGGTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 947 |
| LEAP-Seq percent confirming: | 99.6071 |
| LEAP-Seq n confirming: | 2028 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCTCCTAGTCCTTCAGTG |
| Suggested primer 2: | CGCACACAACTACACACACG |