| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205156 |
| Chromosome: | chromosome 6 |
| Location: | 1990112 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g264050 | (1 of 5) PF04366 - Las17-binding protein actin regulator (Ysc84) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGACAAGCTCTACGGCGAGGGCACCCCAA |
| Internal bar code: | GCAGTTTTTTTTACTATCTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 916 |
| LEAP-Seq percent confirming: | 66.4349 |
| LEAP-Seq n confirming: | 3822 |
| LEAP-Seq n nonconfirming: | 1931 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGATGCTCTGGGTAGGAG |
| Suggested primer 2: | CATTTAATGCAAGCGCAAGA |