| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.205179 |
| Chromosome: | chromosome 17 |
| Location: | 4450064 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732150 | FAP130b,FAP130 | Flagellar Associated Protein 130 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCACTGATACAACAAATACAACTTGAG |
| Internal bar code: | CAAGACCAACTAACAGACCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 535 |
| LEAP-Seq percent confirming: | 99.6461 |
| LEAP-Seq n confirming: | 1408 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATAAAGGTTGGGTGCAAT |
| Suggested primer 2: | TTGAGACGCTCCTTTCGTTT |