| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.205192 |
| Chromosome: | chromosome 14 |
| Location: | 1066463 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614850 | FAP33,ANK17 | Flagellar Associated Protein 33; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCATGTTGCTGCCGACCGACACCTTTGGG |
| Internal bar code: | TTGCTAACTCAAGACTCAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 219 |
| LEAP-Seq percent confirming: | 99.6829 |
| LEAP-Seq n confirming: | 943 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGGATGAGCACTTTGAGT |
| Suggested primer 2: | TTGCGCTGGGCTAGTAGAAT |