| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.205222 |
| Chromosome: | chromosome_17 |
| Location: | 3218454 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g722350 | RABL2A | Small Rab-related GTPase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGGCAAAGCGCAAGCTGCCATTCTTCTTCG |
| Internal bar code: | CACAATCTAGCAGTTTGCTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 150 |
| LEAP-Seq percent confirming: | 94.5283 |
| LEAP-Seq n confirming: | 501 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTCCAAACTCACAGTCC |
| Suggested primer 2: | ACGTCATAGACCAACCCTCG |