Insertion junction: LMJ.RY0402.205224_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.205224
Chromosome:chromosome 9
Location:3191751
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g387356 (1 of 10) PTHR12270//PTHR12270:SF6 - GLYCOSYLTRANSFERASE-RELATED // GLYCOSYLTRANSFERASE-LIKE PROTEIN LARGE CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGCGAGGGCGTTGCCCGGACCGGCGGGG
Internal bar code:ACGGATTCGCAAGGCTGGAAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:537
LEAP-Seq percent confirming:99.4152
LEAP-Seq n confirming:1020
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TACGACAGCAGTTGGTCCAG
Suggested primer 2:CTGAACAATGTCGCACCAAG