| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205228 |
| Chromosome: | chromosome 12 |
| Location: | 3908208 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515950 | (1 of 35) IPR001763 - Rhodanese-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGATCACACGCGCACGATAGGTCGGCGT |
| Internal bar code: | GGCTGCTTATAGAGTTCCGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 442 |
| LEAP-Seq percent confirming: | 99.9086 |
| LEAP-Seq n confirming: | 2186 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTGTCACACACAGAAGT |
| Suggested primer 2: | GTACTTGCGGCACAAGCTG |