Insertion junction: LMJ.RY0402.205256_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.205256
Chromosome:chromosome 4
Location:2894055
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g224500 (1 of 1) 3.6.3.17//3.6.3.44 - Monosaccharide-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCAGCCCGTATGCCTTTGCCGTCCGGTGA
Internal bar code:ACATCGCTCATCCGTGGAGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:646
LEAP-Seq percent confirming:99.8088
LEAP-Seq n confirming:5741
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTTGCCGTCATCAAACAT
Suggested primer 2:AACGCATCCTGCCTTAACAC