| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205278 |
| Chromosome: | chromosome 3 |
| Location: | 6167806 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192201 | PHC51 | Pherophorin-chlamydomonas homolog 51; (1 of 71) PF12499 - Pherophorin (DUF3707) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAAATCAACGTCATGTTATCTAAGTAT |
| Internal bar code: | GTCATTTGGCAACGGATGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1136 |
| LEAP-Seq percent confirming: | 99.1081 |
| LEAP-Seq n confirming: | 5556 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCCCTTCCATCCTCTCC |
| Suggested primer 2: | CTGTTGCGAAAACGACTTGA |