| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.205298 |
| Chromosome: | chromosome 6 |
| Location: | 1802042 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g262450 | VTE7 | (1 of 2) 2.7.1.182 - Phytol kinase; Putative phosphatidate cytidylyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCCTAAGCCGCTCAGCGCTGTACGAAC |
| Internal bar code: | TTGGCCCGAAGCGGCTTGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 669 |
| LEAP-Seq percent confirming: | 99.8314 |
| LEAP-Seq n confirming: | 1184 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGCATTTCATCGCGACAG |
| Suggested primer 2: | TTGTTATACTCCTTGGCCCG |