| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.205299 |
| Chromosome: | chromosome_2 |
| Location: | 5220475 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g105950 | FAP174 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGTTTGCCCTTGGCAGGCTCAGCAGTGCAT |
| Internal bar code: | CCGTGTGCTTTAGCGGAGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 98.524 |
| LEAP-Seq n confirming: | 3204 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGAAGCATTTTGCAATCC |
| Suggested primer 2: | GCTTACGTTACAAGAGCCGC |