| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205310 |
| Chromosome: | chromosome 2 |
| Location: | 6396315 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115500 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCGTCCTGCTTCCAGTCCGTGCTGCAC |
| Internal bar code: | AAGACGACACAGCACTTGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1122 |
| LEAP-Seq percent confirming: | 99.744 |
| LEAP-Seq n confirming: | 7013 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCACAGAACTGCAGGTGG |
| Suggested primer 2: | AAAAGGTGGAGGCAGACCTT |