| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.205314 |
| Chromosome: | chromosome 10 |
| Location: | 2987980 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440750 | TOP3 | Topoisomerase type III, organellar DNA gyrase; (1 of 1) K02470 - DNA gyrase subunit B [EC:5.99.1.3] (gyrB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCATCGTCAGGCCGGGCTGGAATGCAGG |
| Internal bar code: | TATGATTCCACGCGGTAAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 533 |
| LEAP-Seq percent confirming: | 99.5595 |
| LEAP-Seq n confirming: | 2034 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACATGATGTATCCCGCAA |
| Suggested primer 2: | AGCACTTCTGTGTGCAGGTG |