| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205322 |
| Chromosome: | chromosome 4 |
| Location: | 2184130 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219851 | (1 of 2) PF11360 - Protein of unknown function (DUF3110) (DUF3110) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTCAGGACAAAAGAACGGCTATGCAGCG |
| Internal bar code: | CGCGCTCTCTGATGCAGCCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 350 |
| LEAP-Seq percent confirming: | 99.7947 |
| LEAP-Seq n confirming: | 486 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACCCCTCCCATCCATTTT |
| Suggested primer 2: | TTCACCTCACTTGGTGCTTG |