| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.205330 |
| Chromosome: | chromosome_3 |
| Location: | 4059627 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g172550 | PRMT1,PRM1 | Protein-/Histone-arginine N-methyltransferase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCTTGAGTGCAAGCCCAACGCCAAGAACCC |
| Internal bar code: | TGGGTCCTGCGTAGGAAGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1012 |
| LEAP-Seq percent confirming: | 90.6517 |
| LEAP-Seq n confirming: | 1377 |
| LEAP-Seq n nonconfirming: | 142 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACTCGGCTTGCTGTTATG |
| Suggested primer 2: | GTGCCACTACATTACCGCCT |