Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.205330 |
Chromosome: | chromosome_3 |
Location: | 4059628 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g172550 | PRMT1,PRM1 | Protein-/Histone-arginine N-methyltransferase | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGCCGCTGATGGTCTCGTCCTTGCTCGCCA |
Internal bar code: | AAAGCGCAAAAAGTACGAGGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 824 |
LEAP-Seq percent confirming: | 99.5516 |
LEAP-Seq n confirming: | 3774 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGACTCGGCTTGCTGTTATG |
Suggested primer 2: | GTGCCACTACATTACCGCCT |