Insertion junction: LMJ.RY0402.205333_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.205333
Chromosome:chromosome 11
Location:1202082
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g467693 (1 of 5) PF07466 - Protein of unknown function (DUF1517) (DUF1517) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCTGCCCGGGGGCCCCAGCGCTGGCAGC
Internal bar code:GGACCTTCTCGTCTATTTCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:489
LEAP-Seq percent confirming:99.5792
LEAP-Seq n confirming:1420
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGTAGACAGGAGCAAGTC
Suggested primer 2:CCCACCCCCTACACATACAC