Insertion junction: LMJ.RY0402.205372_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.205372
Chromosome:chromosome 9
Location:5908984
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g402515 AMX1 (1 of 2) 1.4.3.21 - Primary-amine oxidase / Copper amine oxidase; Copper amine oxidase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCAGCTGGCACCACGCCCCCCGTAAATAG
Internal bar code:GATGTAATGTCTAACGGGGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:106
LEAP-Seq percent confirming:95.8333
LEAP-Seq n confirming:322
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGCACTCTCATGGACACACA
Suggested primer 2:TGTCATCAACGCTTGCTTTC