Insertion junction: LMJ.RY0402.205373_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.205373
Chromosome:chromosome 7
Location:5170677
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g348400 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCACAACATAAACTGCACCTCTGCCTTG
Internal bar code:AGACCGTCGTAGCGTCACAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:799
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:832
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGCACTGGAAAAGTCTGTCG
Suggested primer 2:CAGGCAGTGAGTCAGATCCA